CULTURE OF PROTOPLASTS FROM CHIMERAL PLANT TISSUE OF NATURE

JAPAN. J. GENETICS Vol. 50, No. 5: 417-420 (1975) SHORT COMMUNICATION CULTURE OF PROTOPLASTS FROM CHIMERAL PLANT TISSUE OF NATURE

There are many chimeral plants induced by somatic cell mutation in nature. It can be expected that protoplasts from somatic cell mutants of chimeral plants are useful materials for plant breeding and genetics, because various mutant plants could be obtained from mutant cells. This investigation was initiated to determine if s'n vitro protoplast curtures could be utilized to obtain mutant plants...

Cell Wall Regeneration around Protoplasts Isolated from Convolvulus Tissue Culture.

Protoplasts of Convolvulus arvensis L. tissue culture regenerated a wall-like structure within 3 days in culture. Although unusually electron dense and atypically amorphous in the electron microscope, this structure could be digested with Myrothecium cellulase but was resistant to protease, a Rohm and Haas pectinase, and a beta-1, 3-exoglucanase just like the original wall. A cytochemical test ...

Secondary Products from Plant Tissue Culture

Isolation of Chloroplasts from Plant Protoplasts.

Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts. The two-part method is a simple, scaled-down, and low-cost procedure that readily pr...

Tissue Culture and Production of Volatile Secondary Metabolites from Callus of Stevia rebaudiana at Different Combination of Plant Growth Regulators

 Stevia rebaudiana Bert (Asteraceae) is herbaceous plant with sweet leaves, which are the source of sweetener products. The present study focuses on effects of different combination of plant growth regulators on callus induction, callus cell growth and regeneration and volatile constituents from callus of stevia in tissue culture condition. The results of present study showed callus formation p...